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wnt8a pcs2  (Addgene inc)


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    Structured Review

    Addgene inc wnt8a pcs2
    (A,C-D,G,I-J,M-N) Phenotypes (A, G) and in situ hybridization of mesendodermal markers (C, myod1 ; D, szl ; I, wnt11b ; J, <t>wnt8a</t> ; M, sox17a ; N, nodal3 . 1 ) in early neurula plate stage (A-D) and mid/early gastrula stages (G-N) in control heterozygotes (+/Z; left-hand panels). (B,E-F,H,K-L,O-P) Phenotypes (B, H) and in situ hybridization of mesendodermal markers (E, myod1 ; F, szl ; K, wnt11b ; L, wnt8a ; O, sox17a ; P, nodal3 . 1 ) in early neurula plate stage (A-D) and mid/early gastrula stages (G-N) in maternal mutant homozygotes (M/Z; right-hand panels). Dorsal, posterior views (A-F); vegetal views (G-P, dorsal towards top). (Q-S) Real-time RT-PCR analysis of myod1 and szl at stage 12/13 (Q), and sia1 (R) and szl at stage 9 and 10.5 (S). Bars in green indicate the samples used for normalization of relative expression; maternal mutants are coloured in cyan. Error bars in (Q) represent standard error of the mean of two biological replicates; representative experiments are shown for (R-S).
    Wnt8a Pcs2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wnt8a pcs2/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    wnt8a pcs2 - by Bioz Stars, 2026-06
    93/100 stars

    Images

    1) Product Images from "Maternal Wnt11b regulates cortical rotation during Xenopus axis formation: analysis of maternal-effect wnt11b mutants"

    Article Title: Maternal Wnt11b regulates cortical rotation during Xenopus axis formation: analysis of maternal-effect wnt11b mutants

    Journal: bioRxiv

    doi: 10.1101/2022.02.02.478872

    (A,C-D,G,I-J,M-N) Phenotypes (A, G) and in situ hybridization of mesendodermal markers (C, myod1 ; D, szl ; I, wnt11b ; J, wnt8a ; M, sox17a ; N, nodal3 . 1 ) in early neurula plate stage (A-D) and mid/early gastrula stages (G-N) in control heterozygotes (+/Z; left-hand panels). (B,E-F,H,K-L,O-P) Phenotypes (B, H) and in situ hybridization of mesendodermal markers (E, myod1 ; F, szl ; K, wnt11b ; L, wnt8a ; O, sox17a ; P, nodal3 . 1 ) in early neurula plate stage (A-D) and mid/early gastrula stages (G-N) in maternal mutant homozygotes (M/Z; right-hand panels). Dorsal, posterior views (A-F); vegetal views (G-P, dorsal towards top). (Q-S) Real-time RT-PCR analysis of myod1 and szl at stage 12/13 (Q), and sia1 (R) and szl at stage 9 and 10.5 (S). Bars in green indicate the samples used for normalization of relative expression; maternal mutants are coloured in cyan. Error bars in (Q) represent standard error of the mean of two biological replicates; representative experiments are shown for (R-S).
    Figure Legend Snippet: (A,C-D,G,I-J,M-N) Phenotypes (A, G) and in situ hybridization of mesendodermal markers (C, myod1 ; D, szl ; I, wnt11b ; J, wnt8a ; M, sox17a ; N, nodal3 . 1 ) in early neurula plate stage (A-D) and mid/early gastrula stages (G-N) in control heterozygotes (+/Z; left-hand panels). (B,E-F,H,K-L,O-P) Phenotypes (B, H) and in situ hybridization of mesendodermal markers (E, myod1 ; F, szl ; K, wnt11b ; L, wnt8a ; O, sox17a ; P, nodal3 . 1 ) in early neurula plate stage (A-D) and mid/early gastrula stages (G-N) in maternal mutant homozygotes (M/Z; right-hand panels). Dorsal, posterior views (A-F); vegetal views (G-P, dorsal towards top). (Q-S) Real-time RT-PCR analysis of myod1 and szl at stage 12/13 (Q), and sia1 (R) and szl at stage 9 and 10.5 (S). Bars in green indicate the samples used for normalization of relative expression; maternal mutants are coloured in cyan. Error bars in (Q) represent standard error of the mean of two biological replicates; representative experiments are shown for (R-S).

    Techniques Used: In Situ Hybridization, Control, Mutagenesis, Quantitative RT-PCR, Expressing



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    wnt8a pcs2  (Addgene inc)
    93
    Addgene inc wnt8a pcs2
    (A,C-D,G,I-J,M-N) Phenotypes (A, G) and in situ hybridization of mesendodermal markers (C, myod1 ; D, szl ; I, wnt11b ; J, <t>wnt8a</t> ; M, sox17a ; N, nodal3 . 1 ) in early neurula plate stage (A-D) and mid/early gastrula stages (G-N) in control heterozygotes (+/Z; left-hand panels). (B,E-F,H,K-L,O-P) Phenotypes (B, H) and in situ hybridization of mesendodermal markers (E, myod1 ; F, szl ; K, wnt11b ; L, wnt8a ; O, sox17a ; P, nodal3 . 1 ) in early neurula plate stage (A-D) and mid/early gastrula stages (G-N) in maternal mutant homozygotes (M/Z; right-hand panels). Dorsal, posterior views (A-F); vegetal views (G-P, dorsal towards top). (Q-S) Real-time RT-PCR analysis of myod1 and szl at stage 12/13 (Q), and sia1 (R) and szl at stage 9 and 10.5 (S). Bars in green indicate the samples used for normalization of relative expression; maternal mutants are coloured in cyan. Error bars in (Q) represent standard error of the mean of two biological replicates; representative experiments are shown for (R-S).
    Wnt8a Pcs2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wnt8a pcs2/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    wnt8a pcs2 - by Bioz Stars, 2026-06
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (A,C-D,G,I-J,M-N) Phenotypes (A, G) and in situ hybridization of mesendodermal markers (C, myod1 ; D, szl ; I, wnt11b ; J, wnt8a ; M, sox17a ; N, nodal3 . 1 ) in early neurula plate stage (A-D) and mid/early gastrula stages (G-N) in control heterozygotes (+/Z; left-hand panels). (B,E-F,H,K-L,O-P) Phenotypes (B, H) and in situ hybridization of mesendodermal markers (E, myod1 ; F, szl ; K, wnt11b ; L, wnt8a ; O, sox17a ; P, nodal3 . 1 ) in early neurula plate stage (A-D) and mid/early gastrula stages (G-N) in maternal mutant homozygotes (M/Z; right-hand panels). Dorsal, posterior views (A-F); vegetal views (G-P, dorsal towards top). (Q-S) Real-time RT-PCR analysis of myod1 and szl at stage 12/13 (Q), and sia1 (R) and szl at stage 9 and 10.5 (S). Bars in green indicate the samples used for normalization of relative expression; maternal mutants are coloured in cyan. Error bars in (Q) represent standard error of the mean of two biological replicates; representative experiments are shown for (R-S).

    Journal: bioRxiv

    Article Title: Maternal Wnt11b regulates cortical rotation during Xenopus axis formation: analysis of maternal-effect wnt11b mutants

    doi: 10.1101/2022.02.02.478872

    Figure Lengend Snippet: (A,C-D,G,I-J,M-N) Phenotypes (A, G) and in situ hybridization of mesendodermal markers (C, myod1 ; D, szl ; I, wnt11b ; J, wnt8a ; M, sox17a ; N, nodal3 . 1 ) in early neurula plate stage (A-D) and mid/early gastrula stages (G-N) in control heterozygotes (+/Z; left-hand panels). (B,E-F,H,K-L,O-P) Phenotypes (B, H) and in situ hybridization of mesendodermal markers (E, myod1 ; F, szl ; K, wnt11b ; L, wnt8a ; O, sox17a ; P, nodal3 . 1 ) in early neurula plate stage (A-D) and mid/early gastrula stages (G-N) in maternal mutant homozygotes (M/Z; right-hand panels). Dorsal, posterior views (A-F); vegetal views (G-P, dorsal towards top). (Q-S) Real-time RT-PCR analysis of myod1 and szl at stage 12/13 (Q), and sia1 (R) and szl at stage 9 and 10.5 (S). Bars in green indicate the samples used for normalization of relative expression; maternal mutants are coloured in cyan. Error bars in (Q) represent standard error of the mean of two biological replicates; representative experiments are shown for (R-S).

    Article Snippet: 1 (from R. Harland; Eco RI/T7), eomes and myod1 (from J. Gurdon; Eco RI/T3 and Bam HI/SP6 respectively), sox17a (from A. Zorn; Asp 718/T3), pitx2c/pbluescript (a gift from M. Blum; Not I/T7) and sizzled/pcs2+ (from M. Kirschner, Addgene plasmid 16688, Bam HI/T3), wnt8a/pcs2+ (XE10, from R. Moon; Addgene plasmid 16865; Bam HI/T3).

    Techniques: In Situ Hybridization, Control, Mutagenesis, Quantitative RT-PCR, Expressing